Citifluor Permafix-1 is an aqueous solution of a chemically modified poly(vinyl alcohol) for use as a permanent mountant. The solution may be used with either Citifluor AF100 or Citifluor AF200 where it is important to reduce the photo bleaching of fluorochromes (see Application Sheets for Citifluor AF100 and Citifluor AF200 for details). A few drops of the solution should be applied to the specimen followed by a cover slip. As the water evaporates cross linking occurs which produces a clear hard robust film which holds the cover slip in place thereby aiding the safe storage of the specimen.
The solutions are of medium viscosity and are water-white in appearance. They contain a small amount of sodium azide to prevent fungal growth. They may be stored at room temperature. The cap of the bottles should always be replaced after use, to prevent evaporation of water which leads to film formation and ultimately to solidification of the product. Samples stored under these conditions for 6 months have been found to exhibit little apparent deterioration e.g. no gel formation.
Citifluor AF100 may used in a ratio of Citifluor Permafix-1 to Citifluor AF100 of 10:1. This additive causes the poly(vinyl alcohol) to set to a very hard film.
Citifluor AF200 should be used in the ratio Citifluor Permafix-1 to Citifluor AF200 of 3:1