/DiATOME Histo Knives
DiATOME Histo Knives2020-11-03T01:53:43+00:00

DiATOME Histo Knives

DiATOME histo, DiATOME histo cryo and DiATOME histo jumbo.
The Diatome histo knife is designed for the sectioning of hard and soft biological and material research specimens, non- embedded or embedded in acrylic or epoxy resins. (Ref. Reymond).

Diatome histo knives can be used on all ultramicrotomes and microtomes with a retraction of the specimen arm in the return phase.

Diatome histo cryo knives are delivered with a boat for wet sectioning using a DMSO/water mixture or in triangular holders for dry sectioning. They are made from a special copper/nickel alloy, which guarantees the best possible thermal conduction.

A crucial step in obtaining ribbons of semithin sections for optical microscopy is the section pickup. The adhesion between semithin sections is relatively weak and the sections often separate from each other during the cutting process or section retrieval. For 3D reconstruction using the Diatome histo jumbo, it is imperative not to lose a single section (Ref. Blumer).

The large jumbo boat fitted to the Diatome histo jumbo and the use of adhesive (Pattex compact by Henkel applied to the upper side of the sample block) allows the user to achieve the following:

  • Easy production of section ribbons (0.5-2μm)
  • No section loss
  • No folding
  • The same orientation of all sections
  • Easy collection of section ribbons
  • Multiple ribbons on one glass slide
  • Perfect for Immuno histo chemistry.


DiATOME histo

Knife angle: 45°
Cutting range: 0.2 – 5μm
Sizes: 4mm, 6mm, 8mm

DiATOME histo cryo

Knife angle: 45°
Cutting range: 0.2 – 5μm
Sizes: 4mm, 6mm

DiATOME histo jumbo

Knife angle: 45°
Cutting range: 0.2 – 5μm
Sizes: 6mm, 8mm

O.L. Reymond: The «semi» diamond knife:
a substitute for glass or conventional diamond knives in the ultramicrotomy of thin and semi-thin sections.
Bas. Appl. Histochem. 30, pp. 487-494, 1986.

M.J.F. Blumer, P. Gahleitner, T. Narzt, C.
Handl, B. Ruthensteiner: Strips of semi-thin sections: an advanced method with a new type of diamond knife.
Journal of Neuroscience Methods 120, pp. 11-16, 2002.
Contact address M.J.F. Blumer: michael.blumer@uibk.ac.at

M.J.F. Blumer: Development of a unique eye: photoreceptors of the pelagic predator Atlanta peroni (Gastropoda, Heteropoda).
Zoomorphology 119, pp. 81-91, 1996.