/DiATOME ultra 35° and DiATOME ultra 45° Room Temperature knives.
DiATOME ultra 35° and DiATOME ultra 45° Room Temperature knives.2021-04-15T03:01:45+00:00

DiATOME ultra 35° and DiATOME ultra 45° Room Temperature Knives

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DiATOME ultra 35°
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In 1989 J. C. Jésior (Ref. Jésior) demonstrated considerably reduced compression, smoother section surfaces and improved structural preservation thanks to the use of our ultra 35° knives.
In the meantime, a large number of scientists have recognized the advantages of 35° knives, in particular for sectioning Lowicryls and non-homogenous specimens, as well as non-decalcified bone, dental materials, etc.

Diatome ultra 35° knives are perfect for sectioning relatively soft materials research specimens including metals and polymers, as well as hard specimens such as semiconductors, superconducting oxides, catalysts, nano-crystalline ceramics, etc (Refs. Mahon, Glanvill, Swab, Quintana, Maniette, Schubert-Bischoff). Diatome ultra 35° knives have demonstrated their usefulness as a standard knife for the majority of applications in both biological and materials research.

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The Diatome ultra 35° knife (in the triangular holder) with a cutting range of 100nm – 2μm is used for dry sectioning of epoxy or acrylic resin embedded biological samples, which need to be investigated by element analysis (Ref. Edelmann) and SIMS
(Ref. Guerquin-Kern). The gliding of the sections on the dry knife surface is facilitated with the use of our Static Line II ionizer.

DiATOME ultra 45°
clip_image006DiATOME ultra 45°The Diatome ultra 45° knife is acknowledged as the appropriate knife angle for routine sectioning of both biological and material research specimens, it represents a balanced compromise between section quality and durability.

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DiATOME ultra jumbo 45° and Diatome ultra jumbo 35º
The Diatome ultra 45º and Diatome ultra 35º are now available with a jumbo boat, which accepts a standard 76mm x 25mm glass slide. Available edge lengths for this version are 2.0mm and 3.0mm.

Specifications

DiATOME ultra 35°

Cutting ranges: 30 – 200nm (standard biological applications) 10 – 30nm (Electron Spectroscopic Imaging ESI, 3D reconstruction, etc) 50nm – 1μm (alternating ultra-thin/semi-thin sectioning) 100nm – 2μm (dry sections for element analysis and SIMS)
Available sizes: 1.5mm, 2.0mm, 2.5mm, 3.0mm, 3.5mm, 4.0mm

DiATOME ultra 45°

Cutting ranges: 30nm – 200nm
Available sizes: 15mm, 2.0mm, 2.5mm. 3.0mm, 3.5mm, 4.0mm

References
J.C. Jésior: Use of low-angle diamond knives leads to improved ultrastructural preservation of ultrathin sections. Scanning Microscopy Supplement 3, pp. 17-153, 1989.
Scanning Microscopy International, Chicago (AMF O’Hare) IL 6066 USA.
L. Edelmann: Freeze-substitution and the preservation of diffusable ions. Journal of Microscopy, Vol. 161, pp. 217-228, 1991.
G. Mahon and T. Malis: Ultramicrotomy of Nano-crystalline Materials. Microscopy Research and Technique, Vol. 31, pp. 267-274, 1995.
S.R. Glanvill: Ultramicrotomy of Semiconductors and Related Materials. Microscopy Research and Technique, Vol. 31, pages 267-274, 1995.
P. Swab and R.E. Klinger: Preparation of multilayer coatings for cross-sectional Microanalysis by Ultramicrotomy. Mat. Res. Soc. Symp. Proc. Vol. 115, pages 229-234, 1989.
P. Swab: Ultra-microscopy of Diamond Films for TEM Cross-Section Analysis. Microscopy Research and Technique, Vol. 31, pp. 308-310, 1995.
C. Quintana: Ultramicrotomy for Cross-sections of Nanostructure. Micron Vol. 28, No. 3, pages 217-219, 1997.
Y. Maniette: Microtomy, a convenient method for preparing TEM samples in ceramic science. Journal of Material Science Letters 9, pages 48-50, 1990.
P. Schubert-Bischoff and T. Krist: Fast cross-sectioning technique for thin films by Ultramicrotomy. Microscopy and Microanalysis, proceedings, page 359, 1997.
J.L. Guerquin-Kern, T.D. Wu, C. Quintana, A. Croisy: Progress in analytical imaging of the cell by dynamic secondary ion mass spectroscopy (SIMS microscopy).
BBA 1724, pp. 228-238, 2005.