Citifluor Aqueous poly
Citifluor Aqueous poly(vinyl pyrrolidone) is very useful as a permanent mountant which possesses some antifadent properties. A few drops of the solution should be applied to the specimen followed by a cover slip. The water evaporates to give a clear film which holds the cover slip in place thereby aiding the safe storage of the specimen.
The solutions are of medium viscosity and are slightly yellow in appearance. They have a pH of ~7. If you require a higher pH you may add a buffer solution such as a tris-solution but this may affect the shelf-life of the solution. The solutions may be stored at room temperature. The cap of the bottles should always be replaced after use, to prevent evaporation of water which leads to film formation and ultimately to solidification of the product. Samples stored under these conditions for 6 months have been found to exhibit little apparent deterioration e.g. no gel formation.
If bleaching of your fluorescent label is a problem use the Citifluor poly(vinyl pyrrolidone) solution containing antifadent.