Citifluor UVM-1 (a UV Mount medium)
Citifluor UVM-1 (a UV Mount medium) is a methacrylate-based solution which on exposure to light hardens to give clear film. It is totally water miscible and therefore can be used with non-dried specimens e.g. specimens that have been washed in buffer solutions. The light source may either be a desk lamp or sunlight! Since the solution cures to give a hard film there is no need to seal the outer edges of an applied coverslip with nail varnish etc.
The preparation does not contain any volatile solvent but, as with all methacrylate solutions, care should be taken not to spill any of the material onto the skin (use appropriate gloves). If fading of fluorochromes is a problem, Citifluor AF100 may be added (ratio of Citifluor UVM-1 to Citifluor AF100 being 10:1)
The solution is of medium viscosity, is water-white in appearance and easy to apply. It must be stored at 10° C or below and out of strong sunlight. The cap of the bottle should always be replaced after use as a matter of good practice and to prevent inadvertent curing.