Sedgewick-Rafter Counting Chamber
The Pyser-SGI Sedgewick Rafter counting cells are used widely in water analysis, culture inspection and for other applications where particles per unit volume in fluid must be determined. The cell can be used with either living or preserved material.
The Pyser-SGI Sedgewick-Rafter Counting Chambers are available in glass and plastic versions. The glass cell is designed for repeated cleaning and re-use, whereas the plastic version is designed for either a single or finite use only. Each Pyser-SGI Sedgewick-Rafter Counting Chamber is supplied with one type S51 cover glass.
The Pyser-SGI Sedgewick-Rafter Counting Chambers consists of a 2mm thick clear base slide (76 x 40 mm), onto which has been built a rectangular chamber. On to the top of this chamber is placed a cover slip. (see Fig A). This Sedgewick-Rafter chamber is 50mm long x 20mm wide and 1mm deep and its base is marked with a grid of one thousand 1mm squares. When filled with liquid and with a cover glass placed over the chamber, a 1ml volume of liquid is trapped. When used under a low magnification light microscope, each of the grid squares equates to 1microlitre (μl) of liquid.
Details of the Pyser-SGI Sedgewick-Rafter Counting Chamber can be found in the Pyser-SGI Counting Chambers catalogue.
Also see Sedgewick-Rafter Technical Notes.